3.25..... setting good for all around riding, if your a guy who rides fairly hard or at least gets on and off the gas a fair amount this will be good and yield off the ruined mag and center cylinder problems in the viper with good premium fuel.
3.5 setting... This is the set up for the slower riders, if you have a wife or kids who ride a viper this is the safe set up. They tend to hold the trottle in the needle section for longer periods of time to run 45-55mph on roads,railroad beds,etc, this where most of the damage always occurs the vipers are way lean right in that area, this 3.5 setting will help cool off the pistons. Still would use premium fuel no matter what.
I use octane boost even in a bone stock sled these days, you have no idea what your getting from that pump, its

junk gas no way around it. So to keep your 2 stroke safe, raise the needles in them vipers and add some booster. No problems to report since doing so in likely more then 75 vipers I have done here... it works! No return burndowns is a good thing!
Just to reitterate the settings:
3.25 setting- 4th groove down from top of needle(non tapered part), both plastic shims on TOP of the clip
3.5 setting- 4th groove down on the needle, 1 plastic shim on TOP of the clip, and 1 plastic shim UNDER the clip, raising the needle up in the slide by the 1 plastic shim.